adenosine a10 receptors activity of entorhinal cortex on amygdala kindled seizures in rat

introduction: the role of adenosine a1 receptors of the entorhinal cortex on amygdaloid kindled seizures was investigated. material and methods: animals were kindled by daily electrical stimulation of amygdala. in the full kindled animals, n6-cyclohexyladenosine (cha; 0.1, 1 and 10 mm), an adenosine a1 receptor agonist, and 8-cyclopenthyle 1,3-dimethylexanthine (cpt), an adenosine a1 receptor antagonist, at concentrations of 1 and 5mm were injected bilaterally into the entorhinal cortex. animals were stimulated at 5, 15, 60 and 120 min post drug infusion and kindling parameters were measured. results: results showed that cha at concentration of 10 mm reduced amygdala after-discharge duration, entrorhinal cortex after-discharge duration (e-add) and stage 5 seizure duration at 5, 15,60 and 120 min post drug injection. it also increased the latency of stage 4 seizure. but, no alteration was observed in seizure stage. at concentrations of 1 and 0.1 mm, cha reduced e-add only at 5 and 15min post drug infusion. bilateral injection of cpt into the entorhinalcortex did not alter seizure parameters. intra-entorhinal cortex injection of cpt (5mm), 5 min before cha (10mm), blocked the anticonvulsant effects of cha. conclusion: these results suggest that the entorhinal cortex has a role in seizure propagation from the amygdala and its adenosine a1 receptors activity have anticonvulsant effects on amygdala kindled seizures.